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Taking this difference into account, the purpose of the immersion liquid is to match (as closely as possible) the refractive index of the glass between which the specimen is mounted, therefore, increasing the amount of light rays which will form the final image. Microscopy image of duodenum captured using Plan Achromat 100x objective lens, with immersion oil. Swing the objective turret ('nosepiece') around between the 40X and the oil immersion objective without engaging the high magnification objective. Ideal for short focused, large magnifications, Oil is an ideal conduit in the preparation of slides because the refractive index is the same or similar as glass. Amongst the discovered species are parasitic worms called We are avid microscope enthusiasts and general explorers of all things tiny. What is the difference between Type A and Type B immersion oil? What is Oil Immersion? And Why is it Important? - GigOptix When the objective is moved to be closer to the slide, the focal plane moves further into the specimen. Which Teeth Are Normally Considered Anodontia? How to Cook With It: This cooking oil has a high smoke point and is great for searing chicken, fish, or tofu. 3 steps of subculture process: o Sterilize the loop. In this short video we'll go over the proper way to use immersion oil, and how to avoid the problems that most frequently ruin microscopes.How To Use A Clini. take the utmost precaution and care when performing a microscope When a wound gets red, swells and heals after a few days, that's known as acute inflammation. When Do We Use the Oil Immersion Objective Lens Never turn your nosepiece back to the 40x objective when using oil or you will get oil all over that objective. Having an immersion liquid in place of the air gap between the front lens of an objective and the cover glass or glass coverslip of a specimen increases the resolution of an objective [3].